Arnold Library

Low freqeuncy production of recombinant subgroup E avian leukosis viruses by uninfected V-15B chicken cells.

Robinson, H L and Eisenman, R and Senior, A and Ripley, S (1979) Low freqeuncy production of recombinant subgroup E avian leukosis viruses by uninfected V-15B chicken cells. Virology, 99 (1). pp. 21-30. ISSN 0042-6822

Full text not available from this repository.
Article URL: http://dx.doi.org/10.1016/0042-6822(79)90033-3

Abstract

15B chicken contains V-15B an allele for the spontaneous production of a noninfectious avian leukosis virus, 15B-ILV. In this paper we present evidence that uninfected 15B cells also produce subgroup E viruses, designated 15B-E viruses. In contrast to 15B-ILV which occurs at an estimated frequency of one event per 103 15B cell days, 15B-E viruses occur at estimated frequencies of one event per 2 × 109 cell days. The frequency of occurrence of 15B-E viruses was affected by the level of expression of V-15B and the presence of endogenous viral alleles which constitutively express subgroup E envelope antigens. The proteins of nine independent isolates of 15B-E viruses were analyzed by SDS-polyacrylamide gel electrophoresis. All isolates had p27 proteins with the distinctive electrophoretic mobility of the p27 of RAV-0, an endogenous virus. Each isolate had characteristic pairs of p19 related proteins which were similar to pairs of p19 related proteins, which are generated by recombination of PR-RSV-C and RAV-0. Since 15B-E isolates occurred in uninfected cells and contained proteins which are characteristic of RAV-0 and recombinants of RAV-0, we suggest that 15B-E viruses are recombinants of endogenous virus information. Since the level of expression of V-15B and the presence of endogenous viral alleles which constitutively express subgroup E envelope antigens affected the frequency of occurrence of 15B-E viruses, we further suggest that 15B-ILV and RNAs which contain information for subgroup E envelope antigens are the parents of 158E viruses.

Item Type: Article
Additional Information: This article is not available online. The link directs to the abstract only.
DOI: 10.1016/0042-6822(79)90033-3
PubMed ID: 91259
Grant Numbers: CA10467, CA23086, P30 12708, CA 20525, CA15704
Keywords or MeSH Headings: Animals; Antigens, Viral; Avian leukosis virus/genetics/growth & development; Cells, Cultured; Chick Embryo; Chickens/microbiology; Genes, Viral; RNA-Directed DNA Polymerase/metabolism; Recombination, Genetic; Viral Proteins/analysis/biosynthesis;
Subjects: Cellular and Organismal Processes > Genetic processes > Recombination
Organisms > Viruses > RNA viruses
Depositing User: Library Staff
Date Deposited: 05 Dec 2008 19:36
Last Modified: 07 May 2010 18:25
URI: http://authors.fhcrc.org/id/eprint/103

Repository Administrators Only

View Item View Item
Fred Hutchinson Cancer Research Center
1100 Fairview Ave. N. PO Box 19024
Seattle, WA 98109

a 501(c)(3) nonprofit organization.

© Terms of Use & Privacy Policy