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Identification of a Rous sarcoma virus transformation-related protein in normal avian and mammalian cells.

Rohrschneider, L R and Eisenman, R N and Leitch, C R (1979) Identification of a Rous sarcoma virus transformation-related protein in normal avian and mammalian cells. Proceedings of the National Academy of Sciences of the United States of America, 76 (9). pp. 4479-4483. ISSN 0027-8424

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Avian sarcoma viruses (ASV) contain a gene (src) whose protein product mediates sarcomagenic transformation. This product is a 60,000-Mr phosphoprotein designated pp60src. We have found that normal uninfected frog, chicken, rat, and human cells contain a 60,000-Mr phosphoprotein related to the product of the ASV src gene and have designated that protein pp60. A phosphoprotein of similar size was not detectable in Drosophila cells. The pp60 proteins were detected by immunoprecipitation with rabbit antitumor serum containing broad spectrum antibodies to pp60src. Peptide maps of [35S]methionine-labeled pp60 and pp60src indicated major similarities as well as some differences in amino acid composition. Peptide maps of the 32P-labeled proteins demonstrated that the phosphopeptides of all endogenous pp60 molecules tested were identical. However, some differences were noted between the phosphopeptide patterns of pp60 and viral pp60src. The kinase activity associated with pp60src was measured in the immunocomplex and resulted in the transfer of radioactive phosphorus from [gamma-32P]ATP to the immunoglobulin heavy chain as well as to an 80,000-Mr phosphoprotein. The pp60 of chicken, rat, and human origin also contained an associated kinase activity. These results are consistent with the notion that the pp60 molecules are the protein products of endogenous sarc sequences found in vertebrate cells.

Item Type: Article or Abstract
Additional Information: This article is freely available in PubMed Central and at the journal's website.
DOI: 10.1073/pnas.76.9.4479
PubMed ID: 92031
PMCID: PMC411600
Grant Numbers: CA 20551, CA 20525
Keywords or MeSH Headings: Amino Acid Sequence; Animals; Anura; Avian Sarcoma Viruses/analysis; Cell Transformation, Viral; Chickens; Epitopes; Humans; Immunologic Techniques; Molecular Weight; Protein Kinases/analysis; Rats; Sarcoma, Experimental/analysis; Viral Proteins/analysis/immunology;
Subjects: Organisms > Viruses > RNA viruses
Molecules > Proteins
Depositing User: Library Staff
Date Deposited: 05 Dec 2008 19:13
Last Modified: 21 May 2010 21:00

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