Groudine, M and Eisenman, R and Weintraub, H (1981) Chromatin structure of endogenous retroviral genes and activation by an inhibitor of DNA methylation. Nature, 292 (5821). pp. 311-317. ISSN 0028-0836
Abstract
The transcriptionally active ev-3 and inactive ev-1 endogenous retrovirus loci in chick cells differ in that ev-3 is undermethylated, preferentially sensitive to DNase I digestion, and contains nuclease hypersensitive sites in each of its two long terminal repeats. Transient exposure of cells to 5-azacytidine, a cytosine analogue which cannot be methylated at the 5 position, results in the hypomethylation and transcriptional activation of ev-1, as well as the acquisition of at least one nuclease-hypersensitive site within the chromosomal domain of ev-1.
| Item Type: | Article or Abstract |
|---|---|
| Additional Information: | This article is available to Nature subscribers only at the link above. |
| DOI: | 10.1038/292311a0 |
| PubMed ID: | 6166864 |
| Keywords or MeSH Headings: | Animals; Azacitidine/pharmacology; Cell Transformation, Viral; Chick Embryo; Chromatin/drug effects/ultrastructure; DNA/genetics; DNA Restriction Enzymes; Deoxyribonuclease I; Deoxyribonucleases; Endonucleases; Methylation; Retroviridae/genetics; Transcription, Genetic/drug effects; |
| Subjects: | Organisms > Viruses > RNA viruses Molecules > Chromosomes > Chromatin |
| Depositing User: | Library Staff |
| Date Deposited: | 05 Dec 2008 00:44 |
| Last Modified: | 07 May 2010 18:27 |
| URI: | http://authors.fhcrc.org/id/eprint/108 |
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