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Nuclear location of the putative transforming protein of avian myelocytomatosis virus.

Abrams, H D and Rohrschneider, L R and Eisenman, R N (1982) Nuclear location of the putative transforming protein of avian myelocytomatosis virus. Cell, 29 (2). pp. 427-439. ISSN 0092-8674

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Article URL: http://dx.doi.org/10.1016/0092-8674(82)90159-3

Abstract

The putative transforming protein of avian myelocytomatosis virus MC29 is a 110,000 dalton (P110gag-myc) polyprotein comprised of sequences derived from both the gag region and the MC29-specific myc region. Two approaches have been taken to determine the location of the MC29 gag-related proteins in transformed cells: subcellular fractionation and immunofluorescence. Analysis of subcellular fractions of MC29-transformed cells by immunoprecipitation indicates that the majority of the gag-myc polyprotein is found in the nuclear fractions of Q8 cells (a nonproducer line of MC29-transformed quail embryo fibroblasts) and nonproducer cells derived from a liver tumor of MC20-infected quail. This is in contrast to the distribution of gag-related helper virus proteins lacking myc, which are found only in nonnuclear fractions of superinfected Q8 cells. The purity of unlabeled nuclei was assessed by electron microscopy and enzyme assays, revealing little contaminating material from other subcellular fractions. Immunofluorescence experiments using monospecific anti-gag serum showed specific, intense immunofluorescence in the nuclei of fixed Q8 cells. In contrast, the majority of P75gag-erb, a candidate transforming protein produced by avian erythroblastosis virus (AEV), is absent from the nuclei of nonproducer AEV-transformed chick embryo fibroblasts. The nuclear association of the MC29 transforming protein may be related to some of the unique properties of MC29-transformed cells.

Item Type: Article
Additional Information: This article is freely available at the journal's website.
DOI: 10.1016/0092-8674(82)90159-3
PubMed ID: 6288259
Keywords or MeSH Headings: Alpharetrovirus/metabolism; Animals; Avian leukosis virus/metabolism; Avian myeloblastosis virus/metabolism; Cell Fractionation; Cell Nucleus/metabolism; Cell Transformation, Viral; Cells, Cultured; Chick Embryo; Defective Viruses/metabolism; Fibroblasts/metabolism; Fluorescent Antibody Technique; Quail; Viral Proteins/immunology/metabolism;
Subjects: Organisms > Viruses > RNA viruses
Molecules > Proteins
Depositing User: Library Staff
Date Deposited: 04 Dec 2008 23:30
Last Modified: 07 May 2010 18:30
URI: http://authors.fhcrc.org/id/eprint/111

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