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Expression of c-myc RNA in bursal lymphoma cell lines: identification of c-myc-encoded proteins by hybrid-selected translation.

Pachl, C and Schubach, W and Eisenman, R N and Linial, M (1983) Expression of c-myc RNA in bursal lymphoma cell lines: identification of c-myc-encoded proteins by hybrid-selected translation. Cell, 33 (2). pp. 335-344. ISSN 0092-8674

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Article URL: http://dx.doi.org/10.1016/0092-8674(83)90415-4

Abstract

We examined expression of the c-myc locus in four cell lines established from bursal lymphomas induced by avian leukosis virus. In all four lines the level of myc-related RNA was elevated. In three lines a majority of the myc-containing RNAs lacked viral-LTR-related sequences, in contrast to results obtained with primary tumors. This suggests that LTR sequences are not required for maintenance of high level c-myc expression. One line, RP9, has a complex pattern of myc RNAs containing LTR sequences, and one of these RNAs is packaged into virions. Using hybrid selection of RNAs with myc DNA, followed by in vitro translation, we detected translation of myc-related proteins from RNA of all four cell lines. The sizes of these proteins differ among the cell lines. The major polypeptides detected were 64, 57, and 54 kilodaltons. Events leading to elevation of c-myc transcription may be accompanied by alterations in mRNA initiation or processing that generate different protein products.

Item Type: Article
Additional Information: This article is freely available at the journal's website.
DOI: 10.1016/0092-8674(83)90415-4
PubMed ID: 6190569
Keywords or MeSH Headings: Animals; Cell Line; DNA Restriction Enzymes/metabolism; Deoxyribonuclease BamHI; Deoxyribonuclease EcoRI; Gene Expression Regulation; Lymphoma/genetics; Molecular Weight; Neoplasm Proteins/biosynthesis; Nucleic Acid Hybridization; Oncogenes; Protein Biosynthesis; RNA/metabolism;
Subjects: Molecules > RNA
Cellular and Organismal Processes > Genetic processes > Translation
Molecules > Genes > Oncogenes
Cellular and Organismal Processes > Genetic processes > Transcription
Depositing User: Library Staff
Date Deposited: 04 Dec 2008 23:23
Last Modified: 07 May 2010 18:31
URI: http://authors.fhcrc.org/id/eprint/112

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