Arnold Library

V-myc- and c-myc-encoded proteins are associated with the nuclear matrix.

Eisenman, R N and Tachibana, C Y and Abrams, H D and Hann, S R (1985) V-myc- and c-myc-encoded proteins are associated with the nuclear matrix. Molecular and cellular biology, 5 (1). pp. 114-126. ISSN 0270-7306

Full text not available from this repository.
Article URL: http://mcb.asm.org/cgi/reprint/5/1/114

Abstract

A series of extraction procedures were applied to avian nuclei which allowed us to define three types of association of v-myc- and c-myc-encoded proteins with nuclei: (i) a major fraction (60 to 90%) which is retained in DNA- and RNA-depleted nuclei after low- and high-salt extraction, (ii) a small fraction (1%) released during nuclease digestion of DNA in intact nuclei in the presence of low-salt buffer, and (iii) a fraction of myc protein (less than 10%) extractable with salt or detergents and found to have affinity for both single- and double-stranded DNA. Immunofluorescence analysis with anti-myc peptide sera on cells extracted sequentially with nucleases and salts confirmed the idea that myc proteins were associated with a complex residual nuclear structure (matrix-lamin fraction) which also contained avian nuclear lamin protein. Dispersal of myc proteins into the cytoplasm was found to occur during mitosis. Both c-myc and v-myc proteins were associated with the matrix-lamin, suggesting that the function of myc may relate to nuclear structural organization.

Item Type: Article
Additional Information: This article is freely available in PubMed Central and at the journal's website.
PubMed ID: 3872410
PMCID: PMC366685
Grant Numbers: CA 20525
Keywords or MeSH Headings: Animals; Cell Nucleus/analysis/ultrastructure; Cell Transformation, Viral; DNA-Binding Proteins/analysis; Deoxyribonucleases/diagnostic use; Gene Products, gag; Lamins; Molecular Weight; Nucleoproteins/metabolism; Oncogenes; Osmolar Concentration; Quail; Ribonucleases/diagnostic use; Viral Proteins/metabolism;
Subjects: Molecules > Molecular structure
Molecules > Proteins
Molecules > Genes > Oncogenes
Depositing User: Library Staff
Date Deposited: 04 Dec 2008 21:57
Last Modified: 21 May 2010 22:32
URI: http://authors.fhcrc.org/id/eprint/116

Repository Administrators Only

View Item View Item
Fred Hutchinson Cancer Research Center
1100 Fairview Ave. N. PO Box 19024
Seattle, WA 98109

a 501(c)(3) nonprofit organization.

© Terms of Use & Privacy Policy