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c-myc oncogene protein synthesis is independent of the cell cycle in human and avian cells.

Hann, S R and Thompson, C B and Eisenman, R N (1985) c-myc oncogene protein synthesis is independent of the cell cycle in human and avian cells. Nature, 314 (6009). pp. 366-369. ISSN 0028-0836

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Several lines of evidence suggest a role for the myc oncogene in cell proliferation. Most recently, mitogenic stimulation of quiescent lymphoid, fibroblast and epithelial cells has been demonstrated to lead to a sharp increase in c-myc RNA levels. To determine how c-myc expression is linked to the cell proliferative cycle, we have used centrifugal elutriation to enrich for populations of avian and human cells at different stages of the cell cycle. Centrifugal elutriation is a counterflow centrifugation method that separates cells on the basis of volume, a parameter correlating well with progression through the cell cycle. Using myc-specific anti-peptide antibodies, we show here that the synthesis, half-life and modification of c-myc proteins are constant throughout the cell cycle of normal and transformed cells.

Item Type: Article or Abstract
Additional Information: This article is available to subscribers only at the link above.
DOI: 10.1038/314366a0
PubMed ID: 3885045
Grant Numbers: CA 20525, CA 2851
Keywords or MeSH Headings: Animals; Cell Cycle; Cell Transformation, Viral; Chick Embryo; Gene Expression Regulation; Humans; Isoelectric Point; Molecular Weight; Neoplasm Proteins/genetics; Oncogenes; Phosphoproteins/biosynthesis/genetics; Proto-Oncogene Proteins c-myc;
Subjects: Molecules > Proteins
Molecules > Genes > Oncogenes
Cellular and Organismal Processes > Cell Physiology > Cell cycle
Depositing User: Library Staff
Date Deposited: 04 Dec 2008 21:46
Last Modified: 07 May 2010 18:33

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