Arnold Library

Expression of the c-myc proto-oncogene during development of Xenopus laevis.

King, M W and Roberts, J M and Eisenman, R N (1986) Expression of the c-myc proto-oncogene during development of Xenopus laevis. Molecular and cellular biology, 6 (12). pp. 4499-4508. ISSN 0270-7306

Full text not available from this repository.
Article URL: http://mcb.asm.org/cgi/reprint/6/12/4499

Abstract

We isolated and characterized Xenopus laevis c-myc cDNAs from an oocyte-specific library. These cDNA clones encompass 2.35 kilobases of the X. laevis c-myc RNA and contain the entire coding domain of 1,257 nucleotides of the 419-amino acid-long X. laevis c-myc protein. The 2.7-kilobase X. laevis c-myc mRNA is expressed in the oocyte, maintained in the egg, and is present throughout the early cleavage stages of embryogenesis. At the time of transcriptional activation in the embryo the c-myc RNA levels show a significant decline and then reaccumulate continuously throughout the remainder of premorphogenic development. At the early neurula stage of embryogenesis the pattern of c-myc RNA expression is elevated in the mesoderm with respect to the endoderm and ectoderm. In the adult X. laevis the c-myc mRNA is expressed in some (e.g., skin, muscle) but not all differentiated tissues. The X. laevis c-myc protein migrates as a doublet of 61,000- and 64,000-dalton species. Both species are phosphorylated in oocytes and somatic cells, exhibit extremely short half-lives of less than 30 min, and are localized to the nuclear fraction of somatic cells. By contrast, the oocyte protein shows both cytoplasmic and germinal vesicle distribution and appears to be stable.

Item Type: Article
Additional Information: This article is freely available in PubMed Central and at the journal's website.
PubMed ID: 3540613
PMCID: PMC367234
Grant Numbers: CA 28151, PF-2646
Keywords or MeSH Headings: Amino Acid Sequence; Animals; Base Sequence; Chickens; Cloning, Molecular; DNA/isolation & purification; Female; Genes; Humans; Oocytes/metabolism; Proto-Oncogene Proteins/genetics; Proto-Oncogene Proteins c-myc; Proto-Oncogenes; Species Specificity; Transcription, Genetic; Xenopus;
Subjects: Cellular and Organismal Processes > Development
Molecules > Genes > Oncogenes
Cellular and Organismal Processes > Genetic processes > Transcription
Depositing User: Library Staff
Date Deposited: 04 Dec 2008 21:24
Last Modified: 21 May 2010 22:34
URI: http://authors.fhcrc.org/id/eprint/119

Repository Administrators Only

View Item View Item
Fred Hutchinson Cancer Research Center
1100 Fairview Ave. N. PO Box 19024
Seattle, WA 98109

a 501(c)(3) nonprofit organization.

© Terms of Use & Privacy Policy