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Drosophila Cdi4 is a p21/p27/p57-like cyclin-dependent kinase inhibitor with specificity for cyclin E complexes.

Finley, Russell L and Barak, Cohen and Brent, Roger (2003) Drosophila Cdi4 is a p21/p27/p57-like cyclin-dependent kinase inhibitor with specificity for cyclin E complexes. Fred Hutchinson Cancer Research Center. (Unpublished)

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Abstract

The eukaryotic cell cycle is controlled by a network of interacting regulatory proteins. We used an interaction mating two-hybrid assay to identify connections within the cell cycle regulatory network in Drosophila. We tested interactions between Drosophila cyclins and a panel of hundreds of previously identified proteins. One of the connections we identified was the interaction between cyclin E and a novel Drosophila protein, Cdi4. Because Cdi4 was originally identified by its ability to interact with a Drosophila cyclin-dependent kinase, the finding that it interacts with cyclin E strengthened the notion that it functions in cell cycle regulation. We show that Cdi4 can inhibit cyclin E function both in a yeast assay and in vitro. In light of these results, our sequence analysis revealed that Cdi4 is a unique member of the p21/p27/p57 family of Cdk inhibitors. Our results demonstrate that interaction mating assays using large informative panels of proteins can aid the analysis of regulatory networks by generating and constraining hypotheses that guide further work.

Item Type: Other
DOI: doi:10.6076/J7WD3XHS
Subjects: Cellular and Organismal Processes > Cell Physiology > Cell cycle
Fred Hutch Divisions: Basic Sciences
Depositing User: Allysha Eyler
Date Deposited: 22 Sep 2016 16:43
Last Modified: 22 Sep 2016 16:51
URI: http://authors.fhcrc.org/id/eprint/1194

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