Cell-to-cell variability in the yeast pheromone response: high throughput screen identifies genes with different effects on transmitted signal and response

Pesce, C. Gustavo and Peria, William and Zdraljevic, Stefan and Rockwell, Dan and Yu, Richard C. and Colman-Lerner, Alejandro and Brent, Roger (2016) Cell-to-cell variability in the yeast pheromone response: high throughput screen identifies genes with different effects on transmitted signal and response. bioRxiv (093187). (Submitted)

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Abstract

Populations of isogenic cells often respond coherently to signals despite differences in protein abundance and cell state. Our previous work in the Saccharomyces cerevisiae pheromone response system (PRS) uncovered processes that reduced cell-to-cell variation in signal and response. To understand these and other processes that controlled variation, we generated a whole-genome collection of haploid strains with deletions in non-essential genes and used high-throughput flow cytometry to screen more than 1000. We identified 50 'variation genes' required for normal cell-to-cell variability in signal and response. Some genes affected only signal variability, signal strength, or system output, defining these quantities as separable 'axes' of system behavior. Two genes affected cytoplasmic microtubule function.

Item Type:	Article or Abstract
DOI:	10.1101/093187
Grant Numbers:	R01 GM097479, R01 GM086615, P50 HG002370
Fred Hutch Divisions:	Basic Sciences
Depositing User:	Ann Marie Clark
Date Deposited:	12 Dec 2016 19:41
Last Modified:	12 Dec 2016 19:41
URI:	http://authors.fhcrc.org/id/eprint/1197

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