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Mad: a heterodimeric partner for Max that antagonizes Myc transcriptional activity.

Ayer, D E and Kretzner, L and Eisenman, R N (1993) Mad: a heterodimeric partner for Max that antagonizes Myc transcriptional activity. Cell, 72 (2). pp. 211-222. ISSN 0092-8674

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Myc family proteins appear to function through heterodimerization with the stable, constitutively expressed bHLH-Zip protein, Max. To determine whether Max mediates the function of regulatory proteins other than Myc, we screened a lambda gt11 expression library with radiolabeled Max protein. One cDNA identified encodes a new member of the bHLH-Zip protein family, Mad. Human Mad protein homodimerizes poorly but binds Max in vitro, forming a sequence-specific DNA binding complex with properties very similar to those of Myc-Max. Both Myc-Max and Mad-Max heterocomplexes are favored over Max homodimers, and, unlike Max homodimers, the DNA binding activity of the heterodimers is unaffected by CKII phosphorylation. Mad does not associate with Myc or with representative bHLH, bZip, or bHLH-Zip proteins. In vivo transactivation assays suggest that Myc-Max and Mad-Max complexes have opposing functions in transcription and that Max plays a central role in this network of transcription factors.

Item Type: Article or Abstract
Additional Information: This article is freely available at the journal's website.
DOI: 10.1016/0092-8674(93)90661-9
PubMed ID: 8425218
Grant Numbers: ROl CA57136
Keywords or MeSH Headings: Amino Acid Sequence; Animals; Baculoviridae/genetics; Base Sequence; Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; Basic-Leucine Zipper Transcription Factors; Binding, Competitive; Casein Kinases; Cell Line; DNA/genetics/metabolism; DNA-Binding Proteins/genetics/metabolism; Genes, myc; Humans; Insects; Macromolecular Substances; Models, Genetic; Molecular Sequence Data; Phosphorylation; Protein Kinases/metabolism; Proto-Oncogene Proteins c-myc/genetics/metabolism; Repressor Proteins; Sequence Homology, Amino Acid; Transcription Factors; Transcription, Genetic; Transfection;
Subjects: Molecules > Proteins
Molecules > Genes > Oncogenes
Cellular and Organismal Processes > Genetic processes > Transcription
Depositing User: Library Staff
Date Deposited: 01 Dec 2008 21:58
Last Modified: 07 May 2010 20:25

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