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Mapping of two genes encoding members of a distinct subfamily of MAX interacting proteins: MAD to human chromosome 2 and mouse chromosome 6, and MXI1 to human chromosome 10 and mouse chromosome 19.

Edelhoff, S and Ayer, D E and Zervos, A S and Steingrímsson, E and Jenkins, N A and Copeland, N G and Eisenman, R N and Brent, R and Disteche, C M (1994) Mapping of two genes encoding members of a distinct subfamily of MAX interacting proteins: MAD to human chromosome 2 and mouse chromosome 6, and MXI1 to human chromosome 10 and mouse chromosome 19. Oncogene, 9 (2). pp. 665-668. ISSN 0950-9232

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Abstract

Both the MAD and the MXI1 genes encode basic-helix-loop-helix-leucine zipper (bHLH-Zip) transcription factors which bind Max in vitro, forming a sequence-specific DNA-binding complex similar to the Myc-Max heterodimer. Mad and Myc compete for binding to Max. In addition, Mad has been shown to act as a transcriptional repressor while Myc appears to function as an activator. Mxi1 also appears to lack a transcriptional activation domain. Therefore, Mxi1 and Mad might antagonize Myc function and are candidate tumor suppressor genes. We report here the mapping of the MAD and MXI1 genes in human and mouse by fluorescence in situ hybridization (FISH) and by recombination mapping. The MAD gene was mapped to human chromosome 2 at band p13 by FISH and to mouse chromosome 6 by meiotic mapping. The MXI1 gene was mapped to human chromosome 10 at band q25 and on mouse chromosome 19 at region D by FISH. There was a second site of hybridization on mouse chromosome 2 at region C, which may represent a pseudogene or a related sequence. The mapping results confirm regions of conservation between human chromosome 2p13 and mouse chromosome 6 and between chromosome 10q25 and mouse chromosome 19D. Human chromosomes 2p13 and 10q25 have been involved in specific tumors where the role of Mad and Mxi1 can now be investigated.

Item Type: Article
PubMed ID: 8290278
Grant Numbers: N01-CO-74101/CO/
Keywords or MeSH Headings: Animals; Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; Basic-Leucine Zipper Transcription Factors; Chromosome Mapping; Chromosomes, Human, Pair 10; Chromosomes, Human, Pair 2; DNA/genetics; DNA-Binding Proteins/genetics/physiology; Gene Expression Regulation/genetics; Genes, Regulator; Genes, Tumor Suppressor; Humans; In Situ Hybridization, Fluorescence; Leucine Zippers; Mice; Transcription Factors/genetics/physiology;
Subjects: Molecules > Proteins > Transcription factors
Molecules > Chromosomes
Research Methodologies > Genomics > Gene mapping
Molecules > Genes > Oncogenes
Depositing User: Library Staff
Date Deposited: 01 Dec 2008 21:00
Last Modified: 21 May 2010 23:08
URI: http://authors.fhcrc.org/id/eprint/148

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