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Pim-1 kinase and p100 cooperate to enhance c-Myb activity.

Leverson, J D and Koskinen, P J and Orrico, F C and Rainio, E M and Jalkanen, K J and Dash, A B and Eisenman, R N and Ness, S A (1998) Pim-1 kinase and p100 cooperate to enhance c-Myb activity. Molecular cell, 2 (4). pp. 417-425. ISSN 1097-2765

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The pim-1 oncogene is regulated by hematopoietic cytokine receptors, encodes a serine/threonine protein kinase, and cooperates with c-myc in lymphoid cell transformation. Using a yeast two-hybrid screen, we found that Pim-1 protein binds to p100, a transcriptional coactivator that interacts with the c-Myb transcription factor. Pim-1 phosphorylated p100 in vitro, formed a stable complex with p100 in animal cells, and functioned downstream of Ras to stimulate c-Myb transcriptional activity in a p100-dependent manner. Thus, Pim-1 and p100 appear to be components of a novel signal transduction pathway affecting c-Myb activity, linking all three to the cytokine-regulated control of hematopoietic cell growth, differentiation, and apoptosis.

Item Type: Article or Abstract
Additional Information: This article is freely available at the journal's website.
DOI: 10.1016/S1097-2765(00)80141-0
PubMed ID: 9809063
Grant Numbers: T32 GM08061, RO1 CA20525, RO1 CA58443
Keywords or MeSH Headings: Acetyltransferases; Animals; Base Sequence; Cells, Cultured; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Neoplastic; Mice; Molecular Sequence Data; Nuclear Proteins/genetics/metabolism; Promoter Regions (Genetics)/physiology; Protein-Serine-Threonine Kinases/genetics/metabolism; Proteins/genetics/metabolism; Proto-Oncogene Proteins/genetics/metabolism; Proto-Oncogene Proteins c-myb; Proto-Oncogene Proteins c-pim-1; Signal Transduction/physiology; Trans-Activators/genetics/metabolism; ras Proteins/metabolism;
Subjects: Cellular and Organismal Processes > Cell Physiology > Cell communication
Molecules > Proteins > Transcription factors
Molecules > Genes > Oncogenes
Depositing User: Library Staff
Date Deposited: 25 Nov 2008 21:27
Last Modified: 07 May 2010 20:46

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