Laherty, C D and Billin, A N and Lavinsky, R M and Yochum, G S and Bush, A C and Sun, J M and Mullen, T M and Davie, J R and Rose, D W and Glass, C K and Rosenfeld, M G and Ayer, D E and Eisenman, R N (1998) SAP30, a component of the mSin3 corepressor complex involved in N-CoR-mediated repression by specific transcription factors. Molecular cell, 2 (1). pp. 33-42. ISSN 1097-2765
Abstract
The transcriptional corepressor mSin3 is found in a large multiprotein complex containing the histone deacetylases HDAC1 and HDAC2, in addition to at least five tightly associated polypeptides. We have cloned and characterized a novel component of the mSin3 complex, SAP30, SAP30 binds to mSin3 and is capable of mediating transcriptional repression via histone deacetylases. SAP30 also binds the N-CoR corepressor and is required for N-CoR-mediated repression by antagonist-bound estrogen receptor and the homeodomain protein Rpx, as well as N-CoR suppression of transactivation by the POU domain protein Pit-1. However, SAP30 is not required for N-CoR-mediated repression by unliganded retinoic acid receptor or thyroid hormone receptor, suggesting that SAP30 is involved in the functional recruitment of the mSin3-histone deacetylase complex to a specific subset of N-CoR corepressor complexes.
| Item Type: | Article or Abstract |
|---|---|
| Additional Information: | This article is freely available from the journal website. |
| DOI: | 10.1016/S1097-2765(00)80111-2 |
| PubMed ID: | 9702189 |
| Keywords or MeSH Headings: | Amino Acid Sequence; Animals; Cell Line, Transformed; DNA-Binding Proteins/physiology; Histone Deacetylases/genetics/physiology; Homeodomain Proteins/physiology; Humans; Kidney; Macromolecular Substances; Mice; Models, Genetic; Molecular Sequence Data; Multiprotein Complexes; Nuclear Proteins/physiology; Receptors, Estrogen/antagonists & inhibitors; Receptors, Retinoic Acid/physiology; Receptors, Thyroid Hormone/physiology; Repressor Proteins/physiology; Saccharomyces cerevisiae Proteins; Transcription Factor Pit-1; Transcription Factors/physiology; Transcription, Genetic; Transfection; |
| Subjects: | Molecules > Proteins > Transcription factors Cellular and Organismal Processes > Genetic processes > Transcription |
| Depositing User: | Library Staff |
| Date Deposited: | 25 Nov 2008 21:15 |
| Last Modified: | 07 May 2010 20:45 |
| URI: | http://authors.fhcrc.org/id/eprint/169 |
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