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Quantitative proteomic analysis of Myc oncoprotein function.

Shiio, Yuzuru and Donohoe, Sam and Yi, Eugene C and Goodlett, David R and Aebersold, Ruedi and Eisenman, Robert N (2002) Quantitative proteomic analysis of Myc oncoprotein function. The EMBO journal, 21 (19). pp. 5088-5096. ISSN 0261-4189

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This study applies a new quantitative proteomics technology to the analysis of the function of the Myc oncoprotein in mammalian cells. Employing isotope-coded affinity tag (ICAT) reagent labeling and tandem mass spectrometry, the global pattern of protein expression in rat myc-null cells was compared with that of myc-plus cells (myc-null cells in which myc has been introduced) to generate a differential protein expression catalog. Expression differences among many functionally related proteins were identified, including reduction of proteases, induction of protein synthesis pathways and upregulation of anabolic enzymes in myc-plus cells, which are predicted to lead to increased cell mass (cell growth). In addition, reduction in the levels of adhesion molecules, actin network proteins and Rho pathway proteins were observed in myc-plus cells, leading to reduced focal adhesions and actin stress fibers as well as altered morphology. These effects are dependent on the highly conserved Myc Box II region. Our results reveal a novel cytoskeletal function for Myc and indicate the feasibility of quantitative whole-proteome analysis in mammalian cells.

Item Type: Article or Abstract
Additional Information: This article is freely available in PubMed Central.
DOI: 10.1093/emboj/cdf525
PubMed ID: 12356725
PMCID: PMC129047
Keywords or MeSH Headings: Animals; Cell Line; Gene Deletion; Genes, myc/genetics; Humans; Indicators and Reagents; Proteome/genetics; Proto-Oncogene Proteins c-myc/genetics; Rats; Recombinant Proteins/genetics; Transfection;
Subjects: Research Methodologies > Proteomics
Molecules > Chromosomes > Chromatin
Molecules > Genes > Oncogenes
Depositing User: Library Staff
Date Deposited: 24 Nov 2008 22:19
Last Modified: 07 May 2010 21:43

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