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Direct activation of RNA polymerase III transcription by c-Myc.

Gomez-Roman, Natividad and Grandori, Carla and Eisenman, Robert N and White, Robert J (2003) Direct activation of RNA polymerase III transcription by c-Myc. Nature, 421 (6920). pp. 290-294. ISSN 0028-0836

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The proto-oncogene product c-Myc has a direct role in both metazoan cell growth and division. RNA polymerase III (pol III) is involved in the generation of transfer RNA and 5S ribosomal RNA, and these molecules must be produced in bulk to meet the need for protein synthesis in growing cells. We demonstrate here that c-Myc binds to TFIIIB, a pol III-specific general transcription factor, and directly activates pol III transcription. Chromatin immunoprecipitation reveals that endogenous c-Myc is present at tRNA and 5S rRNA genes in cultured mammalian cells. These results suggest that activation of pol III may have a role in the ability of c-Myc to stimulate cell growth.

Item Type: Article or Abstract
DOI: 10.1038/nature01327
PubMed ID: 12529648
Keywords or MeSH Headings: Animals; Cell Division; Cell Line; Chromatin/genetics/metabolism; Cyclins/genetics; DNA/genetics/metabolism; Enzyme Activation; Fibroblasts; Gene Deletion; Humans; Precipitin Tests; Protein Binding; Protein Structure, Tertiary; Proto-Oncogene Proteins c-myc/chemistry/genetics/metabolism; RNA Polymerase II/metabolism; RNA Polymerase III/metabolism; RNA, Messenger/genetics/metabolism; RNA, Ribosomal, 5S/genetics; RNA, Transfer/genetics; Rats; Substrate Specificity; Trans-Activation (Genetics); Transcription Factor TFIIIB/metabolism; Transcription, Genetic;
Subjects: Molecules > Proteins > Enzymes
Molecules > RNA
Molecules > Genes > Oncogenes
Cellular and Organismal Processes > Genetic processes > Transcription
Depositing User: Library Staff
Date Deposited: 24 Nov 2008 20:16
Last Modified: 07 May 2010 21:46

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