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Myc-dependent regulation of ribosomal RNA synthesis during Drosophila development.

Grewal, Savraj S and Li, Ling and Orian, Amir and Eisenman, Robert N and Edgar, Bruce A (2005) Myc-dependent regulation of ribosomal RNA synthesis during Drosophila development. Nature cell biology, 7 (3). pp. 295-302. ISSN 1465-7392

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Article URL: http://www.nature.com/ncb/journal/v7/n3/full/ncb12...

Abstract

Regulating ribosome number is thought to control cellular growth. Synthesis of ribosomal RNA (rRNA) is a limiting step in ribosome biogenesis and rates of rRNA synthesis are generally altered depending on the growth status of a cell. Although studies in unicellular systems have addressed the mechanisms by which this occurs, few studies have applied a genetic approach to examine growth-dependent control of rRNA synthesis in metazoans. Here, we show that in Drosophila melanogaster Myc (dMyc) is a regulator of rRNA synthesis. Expression of dMyc is both necessary and sufficient to control rRNA synthesis and ribosome biogenesis during larval development. Stimulation of rRNA synthesis by dMyc is mediated through a rapid, coordinated increase in the levels of the Pol I transcriptional machinery. In addition, the growth effects of dMyc in larval wing imaginal discs require de novo rRNA synthesis. We suggest that during animal development, the control of rRNA synthesis and ribosome biogenesis is an essential Myc function.

Item Type: Article
Additional Information: This article is available to subscribers only at the Article URL above.
DOI: 10.1038/ncb1223
PubMed ID: 15723055
Grant Numbers: R01CA57138, GM51186
Keywords or MeSH Headings: Animals; Blotting, Northern; Blotting, Southern; Cell Nucleolus/metabolism; Drosophila melanogaster; Flow Cytometry; Gene Expression Regulation, Developmental; Green Fluorescent Proteins/metabolism; In Situ Hybridization; Microscopy, Fluorescence; Mitosis; Oligonucleotide Array Sequence Analysis; Proto-Oncogene Proteins c-myc/metabolism; RNA/chemistry; RNA, Ribosomal/chemistry/metabolism; Time Factors;
Subjects: Molecules > RNA
Cellular and Organismal Processes > Development
Molecules > Genes > Oncogenes
Cellular and Organismal Processes > Genetic processes > Transcription
Depositing User: Library Staff
Date Deposited: 12 Dec 2008 21:57
Last Modified: 13 May 2010 22:37
URI: http://authors.fhcrc.org/id/eprint/205

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