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Genomic binding and transcriptional regulation by the Drosophila Myc and Mnt transcription factors.

Orian, A and Grewal, S S and Knoepfler, P S and Edgar, B A and Parkhurst, S M and Eisenman, R N (2005) Genomic binding and transcriptional regulation by the Drosophila Myc and Mnt transcription factors. Cold Spring Harbor symposia on quantitative biology, 70. pp. 299-307. ISSN 0091-7451

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Deregulated expression of members of the myc oncogene family has been linked to the genesis of a wide range of cancers, whereas their normal expression is associated with growth, proliferation, differentiation, and apoptosis. Myc proteins are transcription factors that function within a network of transcriptional activators (Myc) and repressors (Mxd/Mad and Mnt), all of which heterodimerize with the bHLHZ protein Mad and bind E-box sequences in DNA. These transcription factors recruit coactivator or corepressor complexes that in turn modify histones. Myc, Mxd/Max, and Mnt proteins have been thought to act on a specific subset of genes. However, expression array studies and, most recently, genomic binding studies suggest that these proteins exhibit widespread binding across the genome. Here we demonstrate by immunostaining of Drosophila polytene chromosome that Drosophila Myc (dMyc) is associated with multiple euchromatic chromosomal regions. Furthermore, many dMyc-binding regions overlap with regions containing active RNA polymerase II, although dMyc can also be found in regions lacking active polymerase. We also demonstrate that the pattern of dMyc expression in nuclei overlaps with histone markers of active chromatin but not pericentric heterochromatin. dMyc binding is not detected on the X chromosome rDNA cluster (bobbed locus). This is consistent with recent evidence that in Drosophila cells dMyc regulates rRNA transcription indirectly, in contrast to mammalian cells where direct binding of c-Myc to rDNA has been observed. We further show that the dMyc antagonist dMnt inhibits rRNA transcription in the wing disc. Our results support the view that the Myc/Max/Mad network influences transcription on a global scale.

Item Type: Article or Abstract
Additional Information: This article is available to subscribers only via the Article URL above.
DOI: 10.1101/sqb.2005.70.019
PubMed ID: 16869766
Grant Numbers: RO1CA57138, RO1GM073021, RO1 GM51186, CDA 0048/2004C
Keywords or MeSH Headings: Animals; Chromatin/genetics/metabolism; Chromosomes/genetics/metabolism; Drosophila/genetics/metabolism; Drosophila Proteins/genetics/metabolism; Genes, Insect; Proto-Oncogene Proteins c-myc/genetics/metabolism; Transcription Factors/genetics/metabolism; Transcription, Genetic;
Subjects: Molecules > Genes > Oncogenes
Cellular and Organismal Processes > Genetic processes > Transcription
Depositing User: Library Staff
Date Deposited: 17 Dec 2008 00:31
Last Modified: 13 May 2010 21:42

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