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Identification and characterization of SAP25, a novel component of the mSin3 corepressor complex.

Shiio, Yuzuru and Rose, David W and Aur, Radin and Donohoe, Sam and Aebersold, Ruedi and Eisenman, Robert N (2006) Identification and characterization of SAP25, a novel component of the mSin3 corepressor complex. Molecular and cellular biology, 26 (4). pp. 1386-1397. ISSN 0270-7306

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The transcriptional corepressor mSin3 is associated with histone deacetylases (HDACs) and is utilized by many DNA-binding transcriptional repressors. We have cloned and characterized a novel mSin3A-binding protein, SAP25. SAP25 binds to the PAH1 domain of mSin3A, associates with the mSin3A-HDAC complex in vivo, and represses transcription when tethered to DNA. SAP25 is required for mSin3A-mediated, but not N-CoR-mediated, repression. SAP25 is a nucleocytoplasmic shuttling protein, actively exported from the nucleus by a CRM1-dependent mechanism. A fraction of SAP25 is located in promyelocytic leukemia protein (PML) nuclear bodies, and PML induces a striking nuclear accumulation of SAP25. An isotope-coded affinity tag quantitative proteomic analysis of the SAP25 complex revealed that SAP25 is associated with several components of the mSin3 complex, nuclear export machinery, and regulators of transcription and cell cycle. These results suggest that SAP25 is a novel core component of the mSin3 corepressor complex whose subcellular location is regulated by PML.

Item Type: Article or Abstract
Additional Information: This article is freely available in PubMed Central and at the journal's website via the Article URL above.
DOI: 10.1128/MCB.26.4.1386-1397.2006
PubMed ID: 16449650
PMCID: PMC1367179
Grant Numbers: R37CA057138, N01-HV-28179
Keywords or MeSH Headings: Active Transport, Cell Nucleus; Amino Acid Motifs; Amino Acid Sequence; Animals; BALB 3T3 Cells; Carrier Proteins/chemistry/genetics/metabolism; Cell Line; Cloning, Molecular; Hela Cells; Histone Deacetylases/chemistry/genetics/metabolism; Humans; Mice; Molecular Sequence Data; Multiprotein Complexes; Rats; Repressor Proteins/chemistry/genetics/metabolism;
Subjects: Molecules > Proteins > Transcription factors
Cellular and Organismal Processes > Genetic processes > Transcription
Depositing User: Library Staff
Date Deposited: 17 Dec 2008 19:50
Last Modified: 10 May 2010 22:12

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