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Single nucleotide polymorphism-based genome-wide chromosome copy change, loss of heterozygosity, and aneuploidy in Barrett's esophagus neoplastic progression.

Li, Xiaohong and Galipeau, Patricia C and Sanchez, Carissa A and Blount, Patricia L and Maley, Carlo C and Arnaudo, Jessica and Peiffer, Daniel A and Pokholok, Dmitry and Gunderson, Kevin L and Reid, Brian J (2008) Single nucleotide polymorphism-based genome-wide chromosome copy change, loss of heterozygosity, and aneuploidy in Barrett's esophagus neoplastic progression. Cancer prevention research (Philadelphia, Pa.), 1 (6). pp. 413-423. ISSN 1940-6215

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Chromosome copy gain, loss, and loss of heterozygosity (LOH) involving most chromosomes have been reported in many cancers; however, less is known about chromosome instability in premalignant conditions. 17p LOH and DNA content abnormalities have been previously reported to predict progression from Barrett's esophagus (BE) to esophageal adenocarcinoma (EA). Here, we evaluated genome-wide chromosomal instability in multiple stages of BE and EA in whole biopsies. Forty-two patients were selected to represent different stages of progression from BE to EA. Whole BE or EA biopsies were minced, and aliquots were processed for flow cytometry and genotyped with a paired constitutive control for each patient using 33,423 single nucleotide polymorphisms (SNP). Copy gains, losses, and LOH increased in frequency and size between early- and late-stage BE (P < 0.001), with SNP abnormalities increasing from <2% to >30% in early and late stages, respectively. A set of statistically significant events was unique to either early or late, or both, stages, including previously reported and novel abnormalities. The total number of SNP alterations was highly correlated with DNA content aneuploidy and was sensitive and specific to identify patients with concurrent EA (empirical receiver operating characteristic area under the curve = 0.91). With the exception of 9p LOH, most copy gains, losses, and LOH detected in early stages of BE were smaller than those detected in later stages, and few chromosomal events were common in all stages of progression. Measures of chromosomal instability can be quantified in whole biopsies using SNP-based genotyping and have potential to be an integrated platform for cancer risk stratification in BE.

Item Type: Article or Abstract
Additional Information: This article is available to subscribers only at the URL above for the first 12 months post-publication.
DOI: 10.1158/1940-6207.CAPR-08-0121
PubMed ID: 19138988
PMCID: PMC2882787
Grant Numbers: P01 CA091955-06
Keywords or MeSH Headings: Adenocarcinoma/genetics/pathology; Aged; Aneuploidy; Barrett Esophagus/genetics/pathology; Chromosome Aberrations; Chromosomes, Human, Pair 17; Chromosomes, Human, Pair 9; Cross-Sectional Studies; Disease Progression; Esophageal Neoplasms/genetics/pathology; Female; Gene Dosage; Genome, Human; Genome-Wide Association Study/methods; Humans; Loss of Heterozygosity; Male; Middle Aged; Polymorphism, Single Nucleotide;
Subjects: Diseases > Solid tumors > Digestive system cancer
Cellular and Organismal Processes > Genetic processes > Mutation
Cellular and Organismal Processes > Genetic processes > Genomic instability
Research Methodologies > Epidemiology > Risk assessment
Depositing User: Library Staff
Date Deposited: 16 Mar 2009 23:01
Last Modified: 14 Feb 2012 14:42

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