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Generating chimeric zebrafish embryos by transplantation.

Kemp, Hilary A and Carmany-Rampey, Amanda and Moens, Cecilia (2009) Generating chimeric zebrafish embryos by transplantation. Journal of visualized experiments : JoVE (29). p. 1394. ISSN 1940-087X

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Article URL: http://www.jove.com/index/Details.stp?ID=1394

Abstract

One of the most powerful tools used to gain insight into complex developmental processes is the analysis of chimeric embryos. A chimera is defined as an organism that contains cells from more than one animal; mosaics are one type of chimera in which cells from more than one genotype are mixed, usually wild-type and mutant. In the zebrafish, chimeras can be readily made by transplantation of cells from a donor embryo into a host embryo at the appropriate embryonic stage. Labeled donor cells are generated by injection of a lineage marker, such as a fluorescent dye, into the one-cell stage embryo. Labeled donor cells are removed from donor embryos and introduced into unlabeled host embryos using an oil-controlled glass pipette mounted on either a compound or dissecting microscope. Donor cells can in some cases be targeted to a specific region or tissue of the developing blastula or gastrula stage host embryo by choosing a transplantation site in the host embryo based on well-established fate maps.

Item Type: Article
DOI: 10.3791/1394
PubMed ID: 19617875
NIHMSID: NIHMS137341
PMCID: PMC2770904
Grant Numbers: R01 HD037909
Keywords or MeSH Headings: * Animals * Blastula/transplantation * Cell Transplantation/methods* * Embryo Transfer * Embryo, Nonmammalian/cytology * Female * Gastrula/transplantation * Transplantation Chimera/embryology* * Zebrafish/embryology* * Zebrafish/genetics
Subjects: Therapeutics > Transplantation
Organisms > Model organisms
Cellular and Organismal Processes > Development
Depositing User: Library Staff
Date Deposited: 03 Aug 2009 23:20
Last Modified: 14 Feb 2012 14:42
URI: http://authors.fhcrc.org/id/eprint/322

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