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Modulation of human serum glutathione S-transferase-A1/2 concentration by cruciferous vegetables in a controlled feeding study is influenced by GSTM1 and GSTT1 genotypes

Navarro, Sandi L. and Chang, Jyh-Lurn and Peterson, Sabrina and Chen, Chu and King, Irena B. and Schwarz, Yvonne and Li , Shuying S. and Lin, Li and Potter, John D. and Lampe, Johanna W. (2009) Modulation of human serum glutathione S-transferase-A1/2 concentration by cruciferous vegetables in a controlled feeding study is influenced by GSTM1 and GSTT1 genotypes. Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 18 (11). pp. 2974-2978. ISSN 1055-9965

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Glutathione S-transferases (GST) detoxify a wide range of carcinogens. Isothiocyanates (ITC), from cruciferous vegetables, are substrates for, and inducers of GST. GST variants may alter ITC clearance such that response to crucifers varies by genotype. In a randomized cross-over trial, we tested the hypothesis that changes in serum GSTA1/2 concentration in response to cruciferous vegetable feeding depends on GSTM1/GSTT1 genotype. Thirty-three men and 34 women (age 20-40 yr), ate four 14-day controlled diets: basal (vegetable-free), basal supplemented with 2 different doses of crucifers, (single-“dose” and double-“dose”) and single-dose cruciferous-plus-apiaceous vegetables, fed per kg body weight. Fasting bloods from days 0, 7, 11, and 14 of each diet period were analyzed for serum GSTA1/2 by ELISA. GSTA1/2 increased with single- and double-dose cruciferous compared to basal diet (10% and 13%, respectively; P = 0.02 and 0.004), but cruciferous-plus-apiaceous did not differ from basal (P = 0.59). Overall, GSTA1/2 was higher in GSTM1-null/GSTT1-null than GSTM1+/GSTT1+ individuals (4198 ± 338 and 3372 ± 183 pg/ml; P = 0.03). The formal interaction of genotype-by-diet was not statistically significant, but the GSTA1/2 increase during the single-dose cruciferous diet was among GSTM1-null/GSTT1-null individuals (by 28%; P = 0.008), largely explained by GSTM1-null/GSTT1-null men (by 41%; P = 0.01). GSTA1/2 increased during the double-dose cruciferous diet in both GSTM1-null/GSTT1-null men (by 35 %; P = 0.04) and GSTM1+/GSTT1+ men (by 26%; P = 0.01), but not in women. In summary, cruciferous vegetable supplementation increased GSTA1/2, but the effect was most marked in GSTM1-null/GSTT1-null men.

Item Type: Article or Abstract
Additional Information: This article is available to subscribers only via the URL above for the first 12 months following publication.
DOI: 10.1158/1055-9965.EPI-09-0701
PubMed ID: 19900941
PMCID: PMC2777676
Grant Numbers: R01 CA070913-08
Keywords or MeSH Headings: * Adult * Cross-Over Studies * Female * Genotype * Glutathione Transferase/blood* * Glutathione Transferase/genetics* * Homozygote * Humans * Isoenzymes/blood* * Male * Phytotherapy* * Prognosis * Tumor Markers, Biological/genetics* * Vegetables* * Young Adult
Subjects: Molecules > Proteins > Enzymes
Cellular and Organismal Processes > Genetic processes
Health Care > Risk and Preventive Health Services > Diet
Depositing User: Library Staff
Date Deposited: 20 Nov 2009 23:48
Last Modified: 14 Feb 2012 14:42

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