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The mouse Ulnaless mutation deregulates posterior HoxD gene expression and alters appendicular patterning.

Peichel, Catherine L and Prabhakaran, B and Vogt, T F (1997) The mouse Ulnaless mutation deregulates posterior HoxD gene expression and alters appendicular patterning. Development (Cambridge, England), 124 (18). pp. 3481-3492. ISSN 0950-1991

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Article URL: http://dev.biologists.org/cgi/reprint/124/18/3481

Abstract

The semi-dominant mouse mutation Ulnaless alters patterning of the appendicular but not the axial skeleton. Ulnaless forelimbs and hindlimbs have severe reductions of the proximal limb and less severe reductions of the distal limb. Genetic and physical mapping has failed to separate the Ulnaless locus from the HoxD gene cluster (Peichel, C. L., Abbott, C. M. and Vogt, T. F. (1996) Genetics 144, 1757-1767). The Ulnaless limb phenotypes are not recapitulated by targeted mutations in any single HoxD gene, suggesting that Ulnaless may be a gain-of-function mutation in a coding sequence or a regulatory mutation. Deregulation of 5' HoxD gene expression is observed in Ulnaless limb buds. There is ectopic expression of Hoxd-13 and Hoxd-12 in the proximal limb and reduction of Hoxd-13, Hoxd-12 and Hoxd-11 expression in the distal limb. Skeletal reductions in the proximal limb may be a consequence of posterior prevalence, whereby proximal misexpression of Hoxd-13 and Hoxd-12 results in the transcriptional and/or functional inactivation of Hox group 11 genes. The Ulnaless digit phenotypes are attributed to a reduction in the distal expression of Hoxd-13, Hoxd-12, Hoxd-11 and Hoxa-13. In addition, Hoxd-13 expression is reduced in the genital bud, consistent with the observed alterations of the Ulnaless penian bone. No alterations of HoxD expression or skeletal phenotypes were observed in the Ulnaless primary axis. We propose that the Ulnaless mutation alters a cis-acting element that regulates HoxD expression specifically in the appendicular axes of the embryo.

Item Type: Article
Additional Information: This article is available Open Access courtesy of the publisher via the Article URL.
PubMed ID: 9342041
Keywords or MeSH Headings: Animals; Body Patterning; Extremities/embryology; Female; Gene Expression Regulation, Developmental; Gene Targeting; Genes, Homeobox; Genitalia, Female/embryology; Genitalia, Male/embryology; Homeodomain Proteins/genetics; Limb Buds/embryology/metabolism; Limb Deformities, Congenital/embryology/genetics; Male; Mice; Mice, Inbred C57BL; Multigene Family; Mutation; Phenotype; Ulna/abnormalities/embryology;
Subjects: Cellular and Organismal Processes > Genetic processes > Mutation
Cellular and Organismal Processes > Development
Cellular and Organismal Processes > Genetic processes > Transcription
Depositing User: Library Staff
Date Deposited: 23 Sep 2008 16:51
Last Modified: 21 May 2010 16:45
URI: http://authors.fhcrc.org/id/eprint/38

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