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Rho1 regulates signaling events required for proper Drosophila embryonic development.

Magie, Craig R and Parkhurst, Susan M (2005) Rho1 regulates signaling events required for proper Drosophila embryonic development. Developmental biology, 278 (1). pp. 144-54. ISSN 0012-1606

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Article URL: http://dx.doi.org/10.1016/j.ydbio.2004.10.022

Abstract

The Rho small GTPase has been implicated in many cellular processes, including actin cytoskeletal regulation and transcriptional activation. The molecular mechanisms underlying Rho function in many of these processes are not yet clear. Here we report that in Drosophila, reduction of maternal Rho1 compromises signaling pathways consistent with defects in membrane trafficking events. These mutants fail to maintain expression of the segment polarity genes engrailed (en), wingless (wg), and hedgehog (hh), contributing to a segmentation phenotype. Formation of the Wg protein gradient involves the internalization of Wg into vesicles. The number of these Wg-containing vesicles is reduced in maternal Rho1 mutants, suggesting a defect in endocytosis. Consistent with this, stripes of cytoplasmic beta-catenin that accumulate in response to Wg signaling are narrower in these mutants relative to wild type. Additionally, the amount of extracellular Wg protein is reduced in maternal Rho1 mutants, indicating a defect in secretion. Signaling pathways downregulated by endocytosis, such as the epidermal growth factor receptor (EGFR) and Torso pathways, are hyperactivated in maternal Rho1 mutants, consistent with a general role for Rho1 in regulating signaling events governing proper patterning during Drosophila development.

Item Type: Article
DOI: 10.1016/j.ydbio.2004.10.022
PubMed ID: 15649467
NIHMSID: NIHMS299630
PMCID: PMC3125077
Grant Numbers: GM066847
Keywords or MeSH Headings: Animals; Armadillo Domain Proteins; Body Patterning/genetics/physiology; Drosophila/embryology/genetics/metabolism; Drosophila Proteins/genetics/metabolism; Endocytosis/genetics/physiology; Female; Gene Expression Regulation, Developmental; Genes, Insect; Mutation; Proto-Oncogene Proteins/genetics/metabolism; RNA Interference; Signal Transduction; Trans-Activators/genetics/metabolism; Transcription Factors; rho GTP-Binding Proteins/genetics/metabolism;
Subjects: Cellular and Organismal Processes > Cell Physiology > Cell communication
Organisms > Model organisms
Cellular and Organismal Processes > Development
Molecules > Proteins
Depositing User: Library Staff
Date Deposited: 17 Sep 2008 21:35
Last Modified: 14 Feb 2012 14:42
URI: http://authors.fhcrc.org/id/eprint/47

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