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New Genomic Tools for Molecular Studies of Evolutionary Change in Threespine Sticklebacks

Kingsley, David M. and Zhu, Baoli and Osoegawa, Kazutoyo and de Jong, Pieter J and Schein, Jacqueline and Marra, Marco and Peichel, Catherine L and Amemiya, Chris and Schluter, Dolph and Balabhadra, Sarita and Friedlander, Brian and Cha, Yee Man and Dickson, Mark and Grimwood, Jane and Schmutz, Jeremy and Talbot, William S. and Myers, Richard (2004) New Genomic Tools for Molecular Studies of Evolutionary Change in Threespine Sticklebacks. Behaviour, 141 (11-12). 1331-1344(14). ISSN 1568-539X

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The dramatic radiation of sticklebacks in different post-glacial environments provides a unique opportunity to study the molecular mechanisms that underlie rapid evolutionary change in vertebrates. We have developed a number of genomic and genetic tools to facilitate further study of a wide range of morphological, physiological and behavioral traits in sticklebacks. A large collection of microsatellite markers has previously been developed for use in genome-wide linkage mapping of interesting traits in crosses between different stickleback forms. cDNA libraries have been generated and EST sequencing projects have begun to isolate stickleback homologs of developmental control genes. Large insert BAC libraries have been built to compare chromosome regions of interest from both anadromous and freshwater stickleback populations. Large scale fingerprinting of one of these libraries has been used to assemble overlapping contigs of BAC clones for chromosome walking and positional cloning. Together with recent development of methods to make transgenic sticklebacks, these tools should make it possible to identify the molecular basis of many different evolutionary traits in stickleback, and to begin to answer longstanding questions about the numbers and types of mutations that control the appearance of new morphological, physiological, and behavioral traits during vertebrate evolution.

Item Type: Article or Abstract
DOI: 10.1163/1568539042948150
Subjects: Research Methodologies > Genomics
Cellular and Organismal Processes > Evolution
Organisms > Model organisms
Depositing User: Library Staff
Date Deposited: 18 Sep 2008 21:26
Last Modified: 14 Feb 2012 14:42

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