Yeast Isw1p forms two separable complexes in vivo - Supplementary Materials Only

Vary, Jay C and Gangaraju, Vamsi K and Qin, Jun and Landel, Carolyn Church and Kooperberg, Charles and Bartholomew, Blaine and Tsukiyama, Toshio (2003) Yeast Isw1p forms two separable complexes in vivo - Supplementary Materials Only. Molecular and cellular biology, 23 (1). pp. 80-91. ISSN 0270-7306

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Abstract

There are several classes of ATP-dependent chromatin remodeling complexes, which modulate the structure of chromatin to regulate a variety of cellular processes. The budding yeast, Saccharomyces cerevisiae, encodes two ATPases of the ISWI class, Isw1p and Isw2p. Previously Isw1p was shown to copurify with three other proteins. Here we identify these associated proteins and show that Isw1p forms two separable complexes in vivo (designated Isw1a and Isw1b). Biochemical assays revealed that while both have equivalent nucleosome-stimulated ATPase activities, Isw1a and Isw1b differ in their abilities to bind to DNA and nucleosomal substrates, which possibly accounts for differences in specific activities in nucleosomal spacing and sliding. In vivo, the two Isw1 complexes have overlapping functions in transcriptional regulation of some genes yet distinct functions at others. In addition, these complexes show different contributions to cell growth at elevated temperatures.

Item Type:	Article or Abstract
PubMed ID:	12482963
Keywords or MeSH Headings:	Adenosine Triphosphatases/genetics/isolation & purification/metabolism; Amino Acid Motifs; Amino Acid Sequence; DNA, Fungal/metabolism; DNA-Binding Proteins/genetics/isolation & purification/metabolism; Gene Expression Regulation, Fungal; Macromolecular Substances; Molecular Sequence Data; Mutation; Nucleosomes/metabolism; Oligonucleotide Array Sequence Analysis; Protein Isoforms; Saccharomyces cerevisiae/genetics/metabolism; Saccharomyces cerevisiae Proteins/genetics/isolation & purification/metabolism; Sequence Homology, Amino Acid;
Depositing User:	Library Staff
Date Deposited:	24 Oct 2011 19:42
Last Modified:	14 Feb 2012 14:44
URI:	http://authors.fhcrc.org/id/eprint/508

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