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A 13C isotope labeling strategy reveals the influence of insulin signaling on lipogenesis in C. elegans.

Perez, Carissa L and Van Gilst, Marc R (2008) A 13C isotope labeling strategy reveals the influence of insulin signaling on lipogenesis in C. elegans. Cell metabolism, 8 (3). pp. 266-274. ISSN 1932-7420

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Although studies in C. elegans have identified numerous genes involved in fat storage, the next step is to determine how these factors actually affect in vivo lipid metabolism. We have developed a (13)C isotope assay to quantify the contribution of dietary fat absorption and de novo synthesis to fat storage and membrane lipid production in C. elegans, establishing the means by which worms obtain and process fatty acids. We applied this method to characterize how insulin signaling affects lipid physiology. Several long-lived mutations in the insulin receptor gene daf-2 resulted in significantly higher levels of synthesized fats in triglycerides and phospholipids. This elevation of fat synthesis was completely dependent upon daf-16/FoxO. Other long-lived alleles of daf-2 did not increase fat synthesis, however, suggesting that site-specific mutations in the insulin receptor can differentially influence longevity and metabolism, and that elevated lipid synthesis is not required for the longevity of daf-2 mutants.

Item Type: Article or Abstract
DOI: 10.1016/j.cmet.2008.08.007
PubMed ID: 18762027
Grant Numbers: 1-07-JF-72, PHS NRSA T32 GM07270
Keywords or MeSH Headings: Alleles Animals Caenorhabditis elegans/metabolism* Caenorhabditis elegans Proteins/genetics Caenorhabditis elegans Proteins/metabolism Carbon Isotopes Fatty Acids/biosynthesis Fatty Acids/metabolism Insulin/metabolism* Isotope Labeling Lipids/physiology Lipogenesis/physiology* Membrane Lipids/biosynthesis Receptor, Insulin/genetics Receptor, Insulin/metabolism Signal Transduction/physiology* Transcription Factors/metabolism
Subjects: Cellular and Organismal Processes > Cell Physiology > Cell communication
Cellular and Organismal Processes > Metabolism
Molecules > Proteins > Receptors
Depositing User: Library Staff
Date Deposited: 24 Sep 2008 20:28
Last Modified: 18 Sep 2012 14:45

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