Milano, Filippo and Heimfeld, Shelly and Gooley, Ted and Jinneman, Jack and Nicoud, Ian and Delaney, Colleen (2013) Correlation of infused CD3+CD8+ cells with single-donor dominance after double-unit cord blood transplantation. Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation, 19 (1). pp. 156-60. ISSN 1523-6536
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Abstract
Single-donor dominance is observed in the majority of patients following double-unit cord blood transplantation (dCBT); however, the biological basis for this outcome is poorly understood. To investigate the possible influence of specific cell lineages on dominance in dCBT, flow cytometry assessment for CD34(+), CD14(+), CD20(+), CD3(-)CD56(+), CD3(+)CD56(+) (natural killer), and T cell subsets (CD4(+), CD8(+), memory, naïve, and regulatory) was performed on individual units. Subsets were calculated as infused viable cells per kilogram of recipient actual weight. Sixty patients who underwent dCBT were included in the final analysis. Higher CD3(+) cell dose was statistically concordant with the dominant unit in 72% of cases (P = .0006). Further T cell subset analyses showed that dominance was correlated more with the naive CD8(+) cell subset (71% concordance; P = .009) than with the naive CD4(+) cell subset (61% concordance; P = .19). These data indicate that a greater total CD3(+) cell dose, particularly of naïve CD3(+)CD8(+) T cells, may play an important role in determining single-donor dominance after dCBT.
| Item Type: | Article or Abstract |
|---|---|
| Additional Information: | The final published version of this article is available at the publisher's URL listed above. |
| DOI: | 10.1016/j.bbmt.2012.09.004 |
| PubMed ID: | 22989692 |
| NIHMSID: | NIHMS465937 |
| Fred Hutch Divisions: | Clinical Research |
| Depositing User: | Library Staff |
| Date Deposited: | 06 May 2013 17:59 |
| Last Modified: | 06 May 2013 17:59 |
| URI: | http://authors.fhcrc.org/id/eprint/607 |
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