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Identification of C. elegans DAF-12-binding sites, response elements, and target genes.

Shostak, Yuriy and Van Gilst, Marc R and Antebi, Adam and Yamamoto, Keith R (2004) Identification of C. elegans DAF-12-binding sites, response elements, and target genes. Genes & development, 18 (20). pp. 2529-2544. ISSN 0890-9369

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Intracellular receptor DAF-12 regulates dauer formation and developmental age and affects Caenorhabditis elegans lifespan. Genetic analyses place DAF-12 at the convergence of several signal transduction pathways; however, the downstream effectors and the molecular basis for the receptor's multiple physiological outputs are unknown. Beginning with C. elegans genomic DNA, we devised a procedure for multiple rounds of selection and amplification that yielded fragments bearing DAF-12-binding sites. These genomic fragments mediated DAF-12-dependent transcriptional regulation both in Saccharomyces cerevisiae and in C. elegans; that is, they served as functional DAF-12 response elements. We determined that most of the genomic fragments that displayed DAF-12 response element activity in yeast were linked to genes that were regulated by DAF-12 in C. elegans; indeed, the response element-containing fragments typically resided within clusters of DAF-12-regulated genes. DAF-12 target gene regulation was developmental program and stage specific, potentially predicting a fit of these targets into regulatory networks governing aspects of C. elegans reproductive development and dauer formation.

Item Type: Article or Abstract
DOI: 10.1101/gad.1218504
PubMed ID: 15489294
PMCID: PMC529540
Keywords or MeSH Headings: Animals; Base Sequence; Binding Sites/physiology; Caenorhabditis elegans; Caenorhabditis elegans Proteins/genetics/metabolism/physiology; Fluorescence Polarization; Gene Expression Regulation; Genes, Regulator/genetics/physiology; Genes, Reporter/genetics; Green Fluorescent Proteins; Molecular Sequence Data; Oligonucleotides; Receptors, Cytoplasmic and Nuclear/genetics/metabolism/physiology; Response Elements/genetics; Reverse Transcriptase Polymerase Chain Reaction; Saccharomyces cerevisiae; Sequence Analysis, DNA; Signal Transduction/physiology;
Subjects: Cellular and Organismal Processes > Cell Physiology > Cell communication
Cellular and Organismal Processes > Development
Molecules > Proteins > Receptors
Cellular and Organismal Processes > Genetic processes > Transcription
Depositing User: Library Staff
Date Deposited: 30 Sep 2008 18:50
Last Modified: 13 May 2010 21:25

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