Chen, Hung-Ta and Warfield, Linda and Hahn, Steven (2007) The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. Nature structural & molecular biology, 14 (8). pp. 696-703. ISSN 1545-9993
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Abstract
We incorporated the non-natural photoreactive amino acid p-benzoyl-L-phenylalanine (Bpa) into the RNA polymerase II (Pol II) surface surrounding the central cleft formed by the Rpb1 and Rpb2 subunits. Photo-cross-linking of preinitiation complexes (PICs) with these Pol II derivatives and hydroxyl-radical cleavage assays revealed that the TFIIF dimerization domain interacts with the Rpb2 lobe and protrusion domains adjacent to Rpb9, while TFIIE cross-links to the Rpb1 clamp domain on the opposite side of the Pol II central cleft. Mutations in the Rpb2 lobe and protrusion domains alter both Pol II-TFIIF binding and the transcription start site, a phenotype associated with mutations in TFIIF, Rpb9 and TFIIB. Together with previous biochemical and structural studies, these findings illuminate the structural organization of the PIC and the network of protein-protein interactions involved in transcription start site selection.
| Item Type: | Article or Abstract |
|---|---|
| Additional Information: | This article is available to subscribers only via the URL above. |
| DOI: | 10.1038/nsmb1272 |
| PubMed ID: | 17632521 |
| NIHMSID: | NIHMS57570 |
| PMCID: | PMC2483787 |
| Grant Numbers: | R01 GM053451-13 |
| Keywords or MeSH Headings: | Amino Acids/chemistry; Binding Sites; Computer Simulation; Dimerization; Models, Molecular; Mutation; Peptide Mapping; Protein Structure, Tertiary; RNA Polymerase II/chemistry; Saccharomyces cerevisiae/genetics; Substrate Specificity; Transcription Factors, TFII/chemistry; Transcription Initiation Site; |
| Subjects: | Molecules > Proteins > Transcription factors Cellular and Organismal Processes > Genetic processes > Transcription |
| Depositing User: | Library Staff |
| Date Deposited: | 30 Jul 2008 17:54 |
| Last Modified: | 14 Feb 2012 14:42 |
| URI: | http://authors.fhcrc.org/id/eprint/9 |
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