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The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex.

Chen, Hung-Ta and Warfield, Linda and Hahn, Steven (2007) The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. Nature structural & molecular biology, 14 (8). pp. 696-703. ISSN 1545-9993

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Article URL: http://www.nature.com/nsmb/journal/v14/n8/abs/nsmb...

Abstract

We incorporated the non-natural photoreactive amino acid p-benzoyl-L-phenylalanine (Bpa) into the RNA polymerase II (Pol II) surface surrounding the central cleft formed by the Rpb1 and Rpb2 subunits. Photo-cross-linking of preinitiation complexes (PICs) with these Pol II derivatives and hydroxyl-radical cleavage assays revealed that the TFIIF dimerization domain interacts with the Rpb2 lobe and protrusion domains adjacent to Rpb9, while TFIIE cross-links to the Rpb1 clamp domain on the opposite side of the Pol II central cleft. Mutations in the Rpb2 lobe and protrusion domains alter both Pol II-TFIIF binding and the transcription start site, a phenotype associated with mutations in TFIIF, Rpb9 and TFIIB. Together with previous biochemical and structural studies, these findings illuminate the structural organization of the PIC and the network of protein-protein interactions involved in transcription start site selection.

Item Type: Article
Additional Information: This article is available to subscribers only via the URL above.
DOI: 10.1038/nsmb1272
PubMed ID: 17632521
NIHMSID: NIHMS57570
PMCID: PMC2483787
Grant Numbers: R01 GM053451-13
Keywords or MeSH Headings: Amino Acids/chemistry; Binding Sites; Computer Simulation; Dimerization; Models, Molecular; Mutation; Peptide Mapping; Protein Structure, Tertiary; RNA Polymerase II/chemistry; Saccharomyces cerevisiae/genetics; Substrate Specificity; Transcription Factors, TFII/chemistry; Transcription Initiation Site;
Subjects: Molecules > Proteins > Transcription factors
Cellular and Organismal Processes > Genetic processes > Transcription
Depositing User: Library Staff
Date Deposited: 30 Jul 2008 17:54
Last Modified: 14 Feb 2012 14:42
URI: http://authors.fhcrc.org/id/eprint/9

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