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Recombinant avian oncoviruses. II. Alterations in the gag proteins and evidence for intragenic recombination.

Shaikh, R and Linial, M and Brown, S and Sen, A and Eisenman, R (1979) Recombinant avian oncoviruses. II. Alterations in the gag proteins and evidence for intragenic recombination. Virology, 92 (2). pp. 463-481. ISSN 0042-6822

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The internal structural (gag) proteins of recombinant avian oncoviruses selected for the env gene of RAV-O (an endogenous chicken virus) and the src gene for PR-RSV-C were examined. Eight of ten clones of such recombinants were found to synthesize altered gag proteins. The gag proteins of one recombinant clone, PR-E-95c, were examined in more detail by gel electrophoresis and tryptic peptide mapping. These methods have allowed us to distinguish between the gag proteins of the two parental viruses and to determine from which virus the proteins of the recombinant virus were derived. PR-E-95c virions were found to contain p270, an electrophoretically distinguishable variant of p27 which is found in isolates of RAV-0. This recombinant virus also contains p12/15, which is electrophoretically indistinguishable from the p12/15 of both of the parental viruses. However, tryptic peptide analysis of p15 indicates that PR-E-95c has inherited PR-RSV-C-specific p15 sequences. These observations suggest that at least one cross-over has occurred between p15 and p27 in PR-E-95c. A striking difference between the proteins of PR-E-95c virus and those of the parental viruses is that the recombinant lacks polypeptides migrating in the position of p19 and contains two novel polypeptides termed p19α (MW 20,000) and p19β (MW 15,000). Both of these polypeptides are phosphorylated and share antigenic determinants and some tryptic peptides with parental p19. As determined by peptide analysis and radioimmunoassay, these p19-related proteins contain information from both parental viruses, suggesting that PR-E-95c has another cross-over within p19. The altered p19 proteins bind to viral RNA specifically and are associated with genomic RNA in the virion. Neither the stability nor the specific infectivity of the recombinant viruses appears to be significantly affected by the altered proteins.

Item Type: Article or Abstract
Additional Information: This article is not available online. The Article URL directs to the abstract only.
DOI: 10.1016/0042-6822(79)90150-8
PubMed ID: 218355
Grant Numbers: CA18282, CA14142, CA15704, CA20525
Keywords or MeSH Headings: Alpharetrovirus/genetics/physiology; Avian Sarcoma Viruses/genetics; Avian leukosis virus/genetics; Genes, Viral; Phosphoproteins/analysis; RNA, Viral/metabolism; Recombination, Genetic; Viral Proteins/analysis/metabolism;
Subjects: Cellular and Organismal Processes > Genetic processes > Recombination
Organisms > Viruses > RNA viruses
Molecules > Proteins
Depositing User: Library Staff
Date Deposited: 10 Dec 2008 19:28
Last Modified: 07 May 2010 18:22

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