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Cocrystal structure of a class-I preQ1 riboswitch reveals a pseudoknot recognizing an essential hypermodified nucleobase

Klein, Daniel J. and Edwards, Thomas E. and Ferré-D'Amaré, Adrian R. (2009) Cocrystal structure of a class-I preQ1 riboswitch reveals a pseudoknot recognizing an essential hypermodified nucleobase. Nature structural & molecular biology, 16 (3). pp. 343-344. ISSN 1545-9993

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Article URL: http://www.nature.com/nsmb/journal/v16/n3/pdf/nsmb...

Abstract

Riboswitches are mRNA domains that bind metabolites and modulate gene expression in cis. We report cocrystal structures of a remarkably compact riboswitch (34 nucleotides suffice for ligand recognition) from Bacillus subtilis selective for the essential nucleobase preQ1 (7-aminomethyl-7-deazaguanine). These reveal a previously unrecognized pseudoknot fold, and suggest a conserved gene-regulatory mechanism whereby ligand binding promotes sequestration of an RNA segment that otherwise assembles into a transcriptional anti-terminator.

Item Type: Article
Additional Information: This article is available to subscribers only via the URL above
DOI: 10.1038/nsmb.1563
PubMed ID: 19234468
NIHMSID: NIHMS92960
PMCID: PMC2657927
Grant Numbers: HHMI_FERRE-D'AMARE_A, K99 GM084076-01
Keywords or MeSH Headings: Bacillus subtilis/*chemistry/metabolism; Crystallography, X-Ray; Models, Molecular; *Nucleic Acid Conformation; Nucleoside Q/*analogs & derivatives/metabolism; RNA, Bacterial/*chemistry/*metabolism; RNA, Messenger/*chemistry/*metabolism
Subjects: Molecules > RNA
Molecules > Molecular structure
Depositing User: Library Staff
Date Deposited: 06 Feb 2009 23:51
Last Modified: 14 Feb 2012 14:42
URI: http://authors.fhcrc.org/id/eprint/229

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