Klein, Daniel J. and Edwards, Thomas E. and Ferré-D'Amaré, Adrian R. (2009) Cocrystal structure of a class-I preQ1 riboswitch reveals a pseudoknot recognizing an essential hypermodified nucleobase. Nature structural & molecular biology, 16 (3). pp. 343-344. ISSN 1545-9993
![[img]](http://authors.fhcrc.org/style/images/fileicons/application_pdf.png)
| PDF (Complete manuscript) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader 348Kb | |
| PDF (Supplemental materials) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader 569Kb |
Article URL: http://www.nature.com/nsmb/journal/v16/n3/pdf/nsmb.1563.pdf
Riboswitches are mRNA domains that bind metabolites and modulate gene expression in cis. We report cocrystal structures of a remarkably compact riboswitch (34 nucleotides suffice for ligand recognition) from Bacillus subtilis selective for the essential nucleobase preQ1 (7-aminomethyl-7-deazaguanine). These reveal a previously unrecognized pseudoknot fold, and suggest a conserved gene-regulatory mechanism whereby ligand binding promotes sequestration of an RNA segment that otherwise assembles into a transcriptional anti-terminator.
| Item Type: | Article |
|---|---|
| Additional Information: | This article is available to subscribers only via the URL above |
| PubMed ID/Identification Number: | NIHMS: 92960; PMID: 19234468; PMCID: PMC2657927; doi:10.1038/nsmb.1563 |
| Grant Numbers: | HHMI_FERRE-D'AMARE_A, K99 GM084076-01 |
| Keywords or MeSH Headings: | Bacillus subtilis/*chemistry/metabolism; Crystallography, X-Ray; Models, Molecular; *Nucleic Acid Conformation; Nucleoside Q/*analogs & derivatives/metabolism; RNA, Bacterial/*chemistry/*metabolism; RNA, Messenger/*chemistry/*metabolism |
| Subjects: | Molecules > RNA Molecules > Molecular structure |
| Deposited By: | Library Staff |
| Deposited On: | 06 Feb 2009 15:51 |
| Last Modified: | 02 Sep 2009 09:29 |
Repository Staff Only: item control page
