Klein, Daniel J. and Edwards, Thomas E. and Ferré-D'Amaré, Adrian R. (2009) Cocrystal structure of a class-I preQ1 riboswitch reveals a pseudoknot recognizing an essential hypermodified nucleobase. Nature structural & molecular biology, 16 (3). pp. 343-344. ISSN 1545-9993
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Abstract
Riboswitches are mRNA domains that bind metabolites and modulate gene expression in cis. We report cocrystal structures of a remarkably compact riboswitch (34 nucleotides suffice for ligand recognition) from Bacillus subtilis selective for the essential nucleobase preQ1 (7-aminomethyl-7-deazaguanine). These reveal a previously unrecognized pseudoknot fold, and suggest a conserved gene-regulatory mechanism whereby ligand binding promotes sequestration of an RNA segment that otherwise assembles into a transcriptional anti-terminator.
| Item Type: | Article or Abstract |
|---|---|
| Additional Information: | This article is available to subscribers only via the URL above |
| DOI: | 10.1038/nsmb.1563 |
| PubMed ID: | 19234468 |
| NIHMSID: | NIHMS92960 |
| PMCID: | PMC2657927 |
| Grant Numbers: | HHMI_FERRE-D'AMARE_A, K99 GM084076-01 |
| Keywords or MeSH Headings: | Bacillus subtilis/*chemistry/metabolism; Crystallography, X-Ray; Models, Molecular; *Nucleic Acid Conformation; Nucleoside Q/*analogs & derivatives/metabolism; RNA, Bacterial/*chemistry/*metabolism; RNA, Messenger/*chemistry/*metabolism |
| Subjects: | Molecules > RNA Molecules > Molecular structure |
| Depositing User: | Library Staff |
| Date Deposited: | 06 Feb 2009 23:51 |
| Last Modified: | 14 Feb 2012 14:42 |
| URI: | http://authors.fhcrc.org/id/eprint/229 |
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