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ATP-dependent chromatin remodeling shapes the DNA replication landscape.

Vincent, Jack A and Kwong, Tracey J and Tsukiyama, Toshio (2008) ATP-dependent chromatin remodeling shapes the DNA replication landscape. Nature structural & molecular biology, 15 (5). pp. 477-484. ISSN 1545-9985

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Article URL: http://www.nature.com/nsmb/journal/v15/n5/pdf/nsmb...

Abstract

The eukaryotic DNA replication machinery must traverse every nucleosome in the genome during S phase. As nucleosomes are generally inhibitory to DNA-dependent processes, chromatin structure must undergo extensive reorganization to facilitate DNA synthesis. However, the identity of chromatin-remodeling factors involved in replication and how they affect DNA synthesis is largely unknown. Here we show that two highly conserved ATP-dependent chromatin-remodeling complexes in Saccharomyces cerevisiae, Isw2 and Ino80, function in parallel to promote replication fork progression. As a result, Isw2 and Ino80 have especially important roles for replication of late-replicating regions during periods of replication stress. Both Isw2 and Ino80 complexes are enriched at sites of replication, suggesting that these complexes act directly to promote fork progression. These findings identify ATP-dependent chromatin-remodeling complexes that promote DNA replication and define a specific stage of replication that requires remodeling for normal function.

Item Type: Article
DOI: 10.1038/nsmb.1419
PubMed ID: 18408730
NIHMSID: NIHMS104140
PMCID: PMC2678716
Grant Numbers: R01 GM058465-10A1
Keywords or MeSH Headings: Adenosine Triphosphatases/genetics/metabolism; Adenosine Triphosphate/metabolism; Chromatin Assembly and Disassembly; DNA Replication; High Mobility Group Proteins/genetics/metabolism; Methyl Methanesulfonate/pharmacology; Mutation; Nucleosomes/metabolism; S Phase; Saccharomyces cerevisiae/genetics/metabolism; Saccharomyces cerevisiae Proteins/genetics/metabolism; Transcription Factors/genetics/metabolism;
Subjects: Molecules > Proteins > Transcription factors
Molecules > Chromosomes > Chromatin
Cellular and Organismal Processes > Genetic processes > DNA replication
Depositing User: Library Staff
Date Deposited: 20 Mar 2009 19:41
Last Modified: 14 Feb 2012 14:42
URI: http://authors.fhcrc.org/id/eprint/260

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