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Acutely transforming retrovirus expressing Nras generated from HT-1080 fibrosarcoma cells infected with the human retrovirus XMRV.

Metzger, Michael J and Miller, A Dusty (2010) Acutely transforming retrovirus expressing Nras generated from HT-1080 fibrosarcoma cells infected with the human retrovirus XMRV. Journal of virology, 84 (15). pp. 7908-7910. ISSN 1098-5514

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Article URL: http://jvi.asm.org/content/84/15/7908.long

Abstract

Virus from HT-1080 fibrosarcoma cells infected with the human retrovirus XMRV (xenotropic murine leukemia virus-related virus) can induce rare foci of transformation in rat 208F fibroblasts. Characterization of three such foci revealed that one produced an acutely transforming virus at a high titer. The virus consists of a mutant Nras cDNA from the HT-1080 cells inserted into a retroviral vector (added to the HT-1080 cells as a marker for infection) in place of internal vector sequences. These results show that XMRV can generate acutely transforming viruses at a low rate, as is typical of other replication-competent retroviruses, and reveal the potential for transforming virus contamination of retroviral vectors made from transformed cell lines.

Item Type: Article
Additional Information: Copyright © 2010, American Society for Microbiology. All Rights Reserved
DOI: 10.1128/​JVI.00389-10
PubMed ID: 20504941
PMCID: PMC2897646
Depositing User: Library Staff
Date Deposited: 16 Mar 2012 18:25
Last Modified: 16 Mar 2012 18:25
URI: http://authors.fhcrc.org/id/eprint/551

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