Metzger, Michael J and Miller, A Dusty (2010) Acutely transforming retrovirus expressing Nras generated from HT-1080 fibrosarcoma cells infected with the human retrovirus XMRV. Journal of virology, 84 (15). pp. 7908-7910. ISSN 1098-5514
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Abstract
Virus from HT-1080 fibrosarcoma cells infected with the human retrovirus XMRV (xenotropic murine leukemia virus-related virus) can induce rare foci of transformation in rat 208F fibroblasts. Characterization of three such foci revealed that one produced an acutely transforming virus at a high titer. The virus consists of a mutant Nras cDNA from the HT-1080 cells inserted into a retroviral vector (added to the HT-1080 cells as a marker for infection) in place of internal vector sequences. These results show that XMRV can generate acutely transforming viruses at a low rate, as is typical of other replication-competent retroviruses, and reveal the potential for transforming virus contamination of retroviral vectors made from transformed cell lines.
| Item Type: | Article or Abstract |
|---|---|
| Additional Information: | Copyright © 2010, American Society for Microbiology. All Rights Reserved |
| DOI: | 10.1128/JVI.00389-10 |
| PubMed ID: | 20504941 |
| PMCID: | PMC2897646 |
| Depositing User: | Library Staff |
| Date Deposited: | 16 Mar 2012 18:25 |
| Last Modified: | 16 Mar 2012 18:25 |
| URI: | http://authors.fhcrc.org/id/eprint/551 |
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