Arnold Library

Coordination of microtubule and microfilament dynamics by Drosophila Rho1, Spire, and Cappuccino

Rosales-Nieves, Alicia E. and Johndrow, James E. and Keller, Lani C. and Magie, Craig R. and Pinto-Santini, Delia M. and Parkhurst, Susan M. (2006) Coordination of microtubule and microfilament dynamics by Drosophila Rho1, Spire, and Cappuccino. Nature Cell Biology, 8 (4). pp. 367-376. ISSN 1476-4679

[img]
Preview
Text (Manuscript)
RosalesNievesManuscript2006.pdf
Available under License Creative Commons Attribution Non-commercial No Derivatives.

Download (10Mb) | Preview
[img] Video (Movie 1a)
ParkhurstMovie1.mov
Available under License Creative Commons Attribution Non-commercial No Derivatives.

Download (1330Kb)
[img] Video (Movie 1b)
ParkhurstMovie2.mov
Available under License Creative Commons Attribution Non-commercial No Derivatives.

Download (618Kb)
[img] Video (Movie 1c)
ParkhurstMovie3.mov
Available under License Creative Commons Attribution Non-commercial No Derivatives.

Download (2173Kb)
[img] Video (Movie 1d)
ParkhurstMovie4.mov

Download (2700Kb)
[img] Video (Movie 1e)
ParkhurstMovie5.mov
Available under License Creative Commons Attribution Non-commercial No Derivatives.

Download (2049Kb)
[img] Video (Movie 1f)
ParkhurstMovie6.mov
Available under License Creative Commons Attribution Non-commercial No Derivatives.

Download (712Kb)
Article URL: http://www.nature.com/ncb/journal/v8/n4/abs/ncb138...

Abstract

The actin nucleation factors Spire and Cappuccino regulate the onset of ooplasmic streaming in Drosophila1-5. Although this streaming event is microtubule-based, actin assembly is required for its timing. It is not understood how the interaction of microtubules and microfilaments is mediated in this context. Here we demonstrate that Cappuccino and Spire have microtubule and microfilament crosslinking activity. The spire locus encodes several distinct protein isoforms (SpireA, SpireC, and SpireD). SpireD was recently shown to nucleate actin, but the activity of the other isoforms has not been addressed. We find that SpireD does not have crosslinking activity, while SpireC is a potent crosslinker. We show that SpireD binds to Cappuccino and inhibits Factin/ microtubule crosslinking, and activated Rho1 abolishes this inhibition, establishing a mechanistic basis for the regulation of Capu and Spire activity. We propose that Rho1, cappuccino and spire are elements of a conserved developmental cassette that is capable of directly mediating crosstalk between microtubules and microfilaments.

Item Type: Article
DOI: 10.1038/ncb1385
PubMed ID: 16518391
NIHMSID: NIHMS23545
PMCID: PMC1997291
Grant Numbers: GM066847
Keywords or MeSH Headings: Actins/metabolism Animals Cross-Linking Reagents Drosophila Proteins/metabolism* Drosophila melanogaster/cytology Drosophila melanogaster/metabolism* Female Male Microfilament Proteins/metabolism* Microfilaments/metabolism* Microtubules/metabolism* rho GTP-Binding Proteins/metabolism*
Subjects: Cellular and Organismal Processes > Development
Molecules > Proteins > Cytoskeletal proteins
Depositing User: Kristopher Nelson
Date Deposited: 20 Aug 2008 23:27
Last Modified: 14 Feb 2012 14:42
URI: http://authors.fhcrc.org/id/eprint/21

Repository Administrators Only

View Item View Item
Fred Hutchinson Cancer Research Center
1100 Fairview Ave. N. PO Box 19024
Seattle, WA 98109

a 501(c)(3) nonprofit organization.

© Terms of Use & Privacy Policy