Coordination of microtubule and microfilament dynamics by Drosophila Rho1, Spire, and Cappuccino

Rosales-Nieves, Alicia E. and Johndrow, James E. and Keller, Lani C. and Magie, Craig R. and Pinto-Santini, Delia M. and Parkhurst, Susan M. (2006) Coordination of microtubule and microfilament dynamics by Drosophila Rho1, Spire, and Cappuccino. Nature Cell Biology, 8 (4). pp. 367-376. ISSN 1476-4679

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Abstract

The actin nucleation factors Spire and Cappuccino regulate the onset of ooplasmic streaming in Drosophila1-5. Although this streaming event is microtubule-based, actin assembly is required for its timing. It is not understood how the interaction of microtubules and microfilaments is mediated in this context. Here we demonstrate that Cappuccino and Spire have microtubule and microfilament crosslinking activity. The spire locus encodes several distinct protein isoforms (SpireA, SpireC, and SpireD). SpireD was recently shown to nucleate actin, but the activity of the other isoforms has not been addressed. We find that SpireD does not have crosslinking activity, while SpireC is a potent crosslinker. We show that SpireD binds to Cappuccino and inhibits Factin/ microtubule crosslinking, and activated Rho1 abolishes this inhibition, establishing a mechanistic basis for the regulation of Capu and Spire activity. We propose that Rho1, cappuccino and spire are elements of a conserved developmental cassette that is capable of directly mediating crosstalk between microtubules and microfilaments.

Item Type:	Article or Abstract
DOI:	10.1038/ncb1385
PubMed ID:	16518391
NIHMSID:	NIHMS23545
PMCID:	PMC1997291
Grant Numbers:	GM066847
Keywords or MeSH Headings:	Actins/metabolism Animals Cross-Linking Reagents Drosophila Proteins/metabolism* Drosophila melanogaster/cytology Drosophila melanogaster/metabolism* Female Male Microfilament Proteins/metabolism* Microfilaments/metabolism* Microtubules/metabolism* rho GTP-Binding Proteins/metabolism*
Subjects:	Cellular and Organismal Processes > Development Molecules > Proteins > Cytoskeletal proteins
Depositing User:	Users 41 not found.
Date Deposited:	20 Aug 2008 23:27
Last Modified:	14 Feb 2012 14:42
URI:	http://authors.fhcrc.org/id/eprint/21

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