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Supplementary materials - Analysis of gene expression during myc oncogene-induced lymphomagenesis in the bursa of Fabricius.

Neiman, P E and Ruddell, A and Jasoni, C and Loring, G and Thomas, S J and Brandvold, K A and Lee Rm, and Burnside, J and Delrow, J (2001) Supplementary materials - Analysis of gene expression during myc oncogene-induced lymphomagenesis in the bursa of Fabricius. Proceedings of the National Academy of Sciences of the United States of America, 98 (11). pp. 6378-83. ISSN 0027-8424

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Abstract

The transcriptional effects of deregulated myc gene overexpression are implicated in tumorigenesis in a spectrum of experimental and naturally occurring neoplasms. In follicles of the chicken bursa of Fabricius, myc induction of B-cell neoplasia requires a target cell population present during early bursal development and progresses through preneoplastic transformed follicles to metastatic lymphomas. We developed a chicken immune system cDNA microarray to analyze broad changes in gene expression that occur during normal embryonic B-cell development and during myc-induced neoplastic transformation in the bursa. The number of mRNAs showing at least 3-fold change was greater during myc-induced lymphomagenesis than during normal development, and hierarchical cluster analysis of expression patterns revealed that levels of several hundred mRNAs varied in concert with levels of myc overexpression. A set of 41 mRNAs were most consistently elevated in myc-overexpressing preneoplastic and neoplastic cells, most involved in processes thought to be subject to regulation by Myc. The mRNAs for another cluster of genes were overexpressed in neoplasia independent of myc expression level, including a small subset with the expression signature of embryonic bursal lymphocytes. Overexpression of myc, and some of the genes overexpressed with myc, may be important for generation of preneoplastic transformed follicles. However, expression profiles of late metastatic tumors showed a large variation in concert with myc expression levels, and some showed minimal myc overexpression. Therefore, high-level myc overexpression may be more important in the early induction of these lymphomas than in maintenance of late-stage metastases.

Item Type: Article
DOI: 10.1073/pnas.111144898
PubMed ID: 11353853
PMCID: PMC33476
Keywords or MeSH Headings: Animals; Bursa of Fabricius; Chickens; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Genes, myc/physiology; Lymphoma, B-Cell/genetics;
Depositing User: Library Staff
Date Deposited: 22 Dec 2011 20:19
Last Modified: 14 Feb 2012 14:44
URI: http://authors.fhcrc.org/id/eprint/510

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